t cell peptides Search Results


e0089hu  (Shanghai Korain Biotech Co Ltd)
94
Shanghai Korain Biotech Co Ltd e0089hu
E0089hu, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e0089hu/product/Shanghai Korain Biotech Co Ltd
Average 94 stars, based on 1 article reviews
e0089hu - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
interleukin 8 il 8  (MedChemExpress)
93
MedChemExpress interleukin 8 il 8
Anti-IL-8 and anti-VEGFA antibodies partially reverse the ability of MMP28 to promote the migration of TAMs and the polarization of M2 TAMs. A CM from MMP28-overexpressing pancreatic cancer cells was treated with an anti-IL-8 neutralizing antibody (5 μg/mL) or an anti-VEGFA neutralizing antibody (1 μg/mL). The migration level of TAMs was determined by the Transwell migration experiment. Scale bar, 100 μm. B qRT-PCR determination of CD86 and CD163 mRNA expression levels in TAMs cocultured with Vector group of pancreatic cancer cells transfected with empty vector virus, TAMs cocultured with OE-MMP28 cell group, TAMs cocultured with the OE-MMP28 + IgG cell group, TAMs cocultured with OE-MMP28 + Anti-IL-8 cell group, and TAMs cocultured with OE-MMP28 + Anti-VEGFA cell group. C The expression levels of the TAM markers CD86 and CD163 in different coculture groups were determined by immunofluorescence staining. Scale bar, 50 μm. D The proportions of CD11b + CD86 + TAMs and CD11b + CD163 + TAMs in different coculture groups were analysed by flow cytometry. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001
Interleukin 8 Il 8, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/interleukin 8 il 8/product/MedChemExpress
Average 93 stars, based on 1 article reviews
interleukin 8 il 8 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
il 8 h elisa kit  (Boster Bio)
93
Boster Bio il 8 h elisa kit
Anti-IL-8 and anti-VEGFA antibodies partially reverse the ability of MMP28 to promote the migration of TAMs and the polarization of M2 TAMs. A CM from MMP28-overexpressing pancreatic cancer cells was treated with an anti-IL-8 neutralizing antibody (5 μg/mL) or an anti-VEGFA neutralizing antibody (1 μg/mL). The migration level of TAMs was determined by the Transwell migration experiment. Scale bar, 100 μm. B qRT-PCR determination of CD86 and CD163 mRNA expression levels in TAMs cocultured with Vector group of pancreatic cancer cells transfected with empty vector virus, TAMs cocultured with OE-MMP28 cell group, TAMs cocultured with the OE-MMP28 + IgG cell group, TAMs cocultured with OE-MMP28 + Anti-IL-8 cell group, and TAMs cocultured with OE-MMP28 + Anti-VEGFA cell group. C The expression levels of the TAM markers CD86 and CD163 in different coculture groups were determined by immunofluorescence staining. Scale bar, 50 μm. D The proportions of CD11b + CD86 + TAMs and CD11b + CD163 + TAMs in different coculture groups were analysed by flow cytometry. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001
Il 8 H Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/il 8 h elisa kit/product/Boster Bio
Average 93 stars, based on 1 article reviews
il 8 h elisa kit - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
anti cd19  (ProSci Incorporated)
90
ProSci Incorporated anti cd19
Anti-IL-8 and anti-VEGFA antibodies partially reverse the ability of MMP28 to promote the migration of TAMs and the polarization of M2 TAMs. A CM from MMP28-overexpressing pancreatic cancer cells was treated with an anti-IL-8 neutralizing antibody (5 μg/mL) or an anti-VEGFA neutralizing antibody (1 μg/mL). The migration level of TAMs was determined by the Transwell migration experiment. Scale bar, 100 μm. B qRT-PCR determination of CD86 and CD163 mRNA expression levels in TAMs cocultured with Vector group of pancreatic cancer cells transfected with empty vector virus, TAMs cocultured with OE-MMP28 cell group, TAMs cocultured with the OE-MMP28 + IgG cell group, TAMs cocultured with OE-MMP28 + Anti-IL-8 cell group, and TAMs cocultured with OE-MMP28 + Anti-VEGFA cell group. C The expression levels of the TAM markers CD86 and CD163 in different coculture groups were determined by immunofluorescence staining. Scale bar, 50 μm. D The proportions of CD11b + CD86 + TAMs and CD11b + CD163 + TAMs in different coculture groups were analysed by flow cytometry. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001
Anti Cd19, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd19/product/ProSci Incorporated
Average 90 stars, based on 1 article reviews
anti cd19 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
anti transforming growth factor β2  (Boster Bio)
90
Boster Bio anti transforming growth factor β2
Anti-IL-8 and anti-VEGFA antibodies partially reverse the ability of MMP28 to promote the migration of TAMs and the polarization of M2 TAMs. A CM from MMP28-overexpressing pancreatic cancer cells was treated with an anti-IL-8 neutralizing antibody (5 μg/mL) or an anti-VEGFA neutralizing antibody (1 μg/mL). The migration level of TAMs was determined by the Transwell migration experiment. Scale bar, 100 μm. B qRT-PCR determination of CD86 and CD163 mRNA expression levels in TAMs cocultured with Vector group of pancreatic cancer cells transfected with empty vector virus, TAMs cocultured with OE-MMP28 cell group, TAMs cocultured with the OE-MMP28 + IgG cell group, TAMs cocultured with OE-MMP28 + Anti-IL-8 cell group, and TAMs cocultured with OE-MMP28 + Anti-VEGFA cell group. C The expression levels of the TAM markers CD86 and CD163 in different coculture groups were determined by immunofluorescence staining. Scale bar, 50 μm. D The proportions of CD11b + CD86 + TAMs and CD11b + CD163 + TAMs in different coculture groups were analysed by flow cytometry. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001
Anti Transforming Growth Factor β2, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti transforming growth factor β2/product/Boster Bio
Average 90 stars, based on 1 article reviews
anti transforming growth factor β2 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
il 8  (Boster Bio)
89
Boster Bio il 8
Anti-IL-8 and anti-VEGFA antibodies partially reverse the ability of MMP28 to promote the migration of TAMs and the polarization of M2 TAMs. A CM from MMP28-overexpressing pancreatic cancer cells was treated with an anti-IL-8 neutralizing antibody (5 μg/mL) or an anti-VEGFA neutralizing antibody (1 μg/mL). The migration level of TAMs was determined by the Transwell migration experiment. Scale bar, 100 μm. B qRT-PCR determination of CD86 and CD163 mRNA expression levels in TAMs cocultured with Vector group of pancreatic cancer cells transfected with empty vector virus, TAMs cocultured with OE-MMP28 cell group, TAMs cocultured with the OE-MMP28 + IgG cell group, TAMs cocultured with OE-MMP28 + Anti-IL-8 cell group, and TAMs cocultured with OE-MMP28 + Anti-VEGFA cell group. C The expression levels of the TAM markers CD86 and CD163 in different coculture groups were determined by immunofluorescence staining. Scale bar, 50 μm. D The proportions of CD11b + CD86 + TAMs and CD11b + CD163 + TAMs in different coculture groups were analysed by flow cytometry. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001
Il 8, supplied by Boster Bio, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/il 8/product/Boster Bio
Average 89 stars, based on 1 article reviews
il 8 - by Bioz Stars, 2026-02
89/100 stars
  Buy from Supplier
cd4 helper t cells  (ProSci Incorporated)
93
ProSci Incorporated cd4 helper t cells
Anti-IL-8 and anti-VEGFA antibodies partially reverse the ability of MMP28 to promote the migration of TAMs and the polarization of M2 TAMs. A CM from MMP28-overexpressing pancreatic cancer cells was treated with an anti-IL-8 neutralizing antibody (5 μg/mL) or an anti-VEGFA neutralizing antibody (1 μg/mL). The migration level of TAMs was determined by the Transwell migration experiment. Scale bar, 100 μm. B qRT-PCR determination of CD86 and CD163 mRNA expression levels in TAMs cocultured with Vector group of pancreatic cancer cells transfected with empty vector virus, TAMs cocultured with OE-MMP28 cell group, TAMs cocultured with the OE-MMP28 + IgG cell group, TAMs cocultured with OE-MMP28 + Anti-IL-8 cell group, and TAMs cocultured with OE-MMP28 + Anti-VEGFA cell group. C The expression levels of the TAM markers CD86 and CD163 in different coculture groups were determined by immunofluorescence staining. Scale bar, 50 μm. D The proportions of CD11b + CD86 + TAMs and CD11b + CD163 + TAMs in different coculture groups were analysed by flow cytometry. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001
Cd4 Helper T Cells, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd4 helper t cells/product/ProSci Incorporated
Average 93 stars, based on 1 article reviews
cd4 helper t cells - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
human tgf β2 picokinetm elisa kit  (Boster Bio)
91
Boster Bio human tgf β2 picokinetm elisa kit
Anti-IL-8 and anti-VEGFA antibodies partially reverse the ability of MMP28 to promote the migration of TAMs and the polarization of M2 TAMs. A CM from MMP28-overexpressing pancreatic cancer cells was treated with an anti-IL-8 neutralizing antibody (5 μg/mL) or an anti-VEGFA neutralizing antibody (1 μg/mL). The migration level of TAMs was determined by the Transwell migration experiment. Scale bar, 100 μm. B qRT-PCR determination of CD86 and CD163 mRNA expression levels in TAMs cocultured with Vector group of pancreatic cancer cells transfected with empty vector virus, TAMs cocultured with OE-MMP28 cell group, TAMs cocultured with the OE-MMP28 + IgG cell group, TAMs cocultured with OE-MMP28 + Anti-IL-8 cell group, and TAMs cocultured with OE-MMP28 + Anti-VEGFA cell group. C The expression levels of the TAM markers CD86 and CD163 in different coculture groups were determined by immunofluorescence staining. Scale bar, 50 μm. D The proportions of CD11b + CD86 + TAMs and CD11b + CD163 + TAMs in different coculture groups were analysed by flow cytometry. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001
Human Tgf β2 Picokinetm Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human tgf β2 picokinetm elisa kit/product/Boster Bio
Average 91 stars, based on 1 article reviews
human tgf β2 picokinetm elisa kit - by Bioz Stars, 2026-02
91/100 stars
  Buy from Supplier
anti human cd3  (ProSci Incorporated)
90
ProSci Incorporated anti human cd3
Anti-IL-8 and anti-VEGFA antibodies partially reverse the ability of MMP28 to promote the migration of TAMs and the polarization of M2 TAMs. A CM from MMP28-overexpressing pancreatic cancer cells was treated with an anti-IL-8 neutralizing antibody (5 μg/mL) or an anti-VEGFA neutralizing antibody (1 μg/mL). The migration level of TAMs was determined by the Transwell migration experiment. Scale bar, 100 μm. B qRT-PCR determination of CD86 and CD163 mRNA expression levels in TAMs cocultured with Vector group of pancreatic cancer cells transfected with empty vector virus, TAMs cocultured with OE-MMP28 cell group, TAMs cocultured with the OE-MMP28 + IgG cell group, TAMs cocultured with OE-MMP28 + Anti-IL-8 cell group, and TAMs cocultured with OE-MMP28 + Anti-VEGFA cell group. C The expression levels of the TAM markers CD86 and CD163 in different coculture groups were determined by immunofluorescence staining. Scale bar, 50 μm. D The proportions of CD11b + CD86 + TAMs and CD11b + CD163 + TAMs in different coculture groups were analysed by flow cytometry. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001
Anti Human Cd3, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti human cd3/product/ProSci Incorporated
Average 90 stars, based on 1 article reviews
anti human cd3 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
lef  (ProSci Incorporated)
86
ProSci Incorporated lef
Anti-IL-8 and anti-VEGFA antibodies partially reverse the ability of MMP28 to promote the migration of TAMs and the polarization of M2 TAMs. A CM from MMP28-overexpressing pancreatic cancer cells was treated with an anti-IL-8 neutralizing antibody (5 μg/mL) or an anti-VEGFA neutralizing antibody (1 μg/mL). The migration level of TAMs was determined by the Transwell migration experiment. Scale bar, 100 μm. B qRT-PCR determination of CD86 and CD163 mRNA expression levels in TAMs cocultured with Vector group of pancreatic cancer cells transfected with empty vector virus, TAMs cocultured with OE-MMP28 cell group, TAMs cocultured with the OE-MMP28 + IgG cell group, TAMs cocultured with OE-MMP28 + Anti-IL-8 cell group, and TAMs cocultured with OE-MMP28 + Anti-VEGFA cell group. C The expression levels of the TAM markers CD86 and CD163 in different coculture groups were determined by immunofluorescence staining. Scale bar, 50 μm. D The proportions of CD11b + CD86 + TAMs and CD11b + CD163 + TAMs in different coculture groups were analysed by flow cytometry. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001
Lef, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lef/product/ProSci Incorporated
Average 86 stars, based on 1 article reviews
lef - by Bioz Stars, 2026-02
86/100 stars
  Buy from Supplier
macrospin columns  (Chem Impex International)
95
Chem Impex International macrospin columns
Anti-IL-8 and anti-VEGFA antibodies partially reverse the ability of MMP28 to promote the migration of TAMs and the polarization of M2 TAMs. A CM from MMP28-overexpressing pancreatic cancer cells was treated with an anti-IL-8 neutralizing antibody (5 μg/mL) or an anti-VEGFA neutralizing antibody (1 μg/mL). The migration level of TAMs was determined by the Transwell migration experiment. Scale bar, 100 μm. B qRT-PCR determination of CD86 and CD163 mRNA expression levels in TAMs cocultured with Vector group of pancreatic cancer cells transfected with empty vector virus, TAMs cocultured with OE-MMP28 cell group, TAMs cocultured with the OE-MMP28 + IgG cell group, TAMs cocultured with OE-MMP28 + Anti-IL-8 cell group, and TAMs cocultured with OE-MMP28 + Anti-VEGFA cell group. C The expression levels of the TAM markers CD86 and CD163 in different coculture groups were determined by immunofluorescence staining. Scale bar, 50 μm. D The proportions of CD11b + CD86 + TAMs and CD11b + CD163 + TAMs in different coculture groups were analysed by flow cytometry. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001
Macrospin Columns, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/macrospin columns/product/Chem Impex International
Average 95 stars, based on 1 article reviews
macrospin columns - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
t-cnrp1 peptide (tat cell penetrating peptide sequence: ygrkkrrqrrr fused to cnrp1: hvtegsgryiprkpf)  (GenScript corporation)
90
GenScript corporation t-cnrp1 peptide (tat cell penetrating peptide sequence: ygrkkrrqrrr fused to cnrp1: hvtegsgryiprkpf)
Anti-IL-8 and anti-VEGFA antibodies partially reverse the ability of MMP28 to promote the migration of TAMs and the polarization of M2 TAMs. A CM from MMP28-overexpressing pancreatic cancer cells was treated with an anti-IL-8 neutralizing antibody (5 μg/mL) or an anti-VEGFA neutralizing antibody (1 μg/mL). The migration level of TAMs was determined by the Transwell migration experiment. Scale bar, 100 μm. B qRT-PCR determination of CD86 and CD163 mRNA expression levels in TAMs cocultured with Vector group of pancreatic cancer cells transfected with empty vector virus, TAMs cocultured with OE-MMP28 cell group, TAMs cocultured with the OE-MMP28 + IgG cell group, TAMs cocultured with OE-MMP28 + Anti-IL-8 cell group, and TAMs cocultured with OE-MMP28 + Anti-VEGFA cell group. C The expression levels of the TAM markers CD86 and CD163 in different coculture groups were determined by immunofluorescence staining. Scale bar, 50 μm. D The proportions of CD11b + CD86 + TAMs and CD11b + CD163 + TAMs in different coculture groups were analysed by flow cytometry. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001
T Cnrp1 Peptide (Tat Cell Penetrating Peptide Sequence: Ygrkkrrqrrr Fused To Cnrp1: Hvtegsgryiprkpf), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t-cnrp1 peptide (tat cell penetrating peptide sequence: ygrkkrrqrrr fused to cnrp1: hvtegsgryiprkpf)/product/GenScript corporation
Average 90 stars, based on 1 article reviews
t-cnrp1 peptide (tat cell penetrating peptide sequence: ygrkkrrqrrr fused to cnrp1: hvtegsgryiprkpf) - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


Anti-IL-8 and anti-VEGFA antibodies partially reverse the ability of MMP28 to promote the migration of TAMs and the polarization of M2 TAMs. A CM from MMP28-overexpressing pancreatic cancer cells was treated with an anti-IL-8 neutralizing antibody (5 μg/mL) or an anti-VEGFA neutralizing antibody (1 μg/mL). The migration level of TAMs was determined by the Transwell migration experiment. Scale bar, 100 μm. B qRT-PCR determination of CD86 and CD163 mRNA expression levels in TAMs cocultured with Vector group of pancreatic cancer cells transfected with empty vector virus, TAMs cocultured with OE-MMP28 cell group, TAMs cocultured with the OE-MMP28 + IgG cell group, TAMs cocultured with OE-MMP28 + Anti-IL-8 cell group, and TAMs cocultured with OE-MMP28 + Anti-VEGFA cell group. C The expression levels of the TAM markers CD86 and CD163 in different coculture groups were determined by immunofluorescence staining. Scale bar, 50 μm. D The proportions of CD11b + CD86 + TAMs and CD11b + CD163 + TAMs in different coculture groups were analysed by flow cytometry. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: MMP28 recruits M2-type tumor-associated macrophages through MAPK/JNK signaling pathway-dependent cytokine secretion to promote the malignant progression of pancreatic cancer

doi: 10.1186/s13046-025-03321-x

Figure Lengend Snippet: Anti-IL-8 and anti-VEGFA antibodies partially reverse the ability of MMP28 to promote the migration of TAMs and the polarization of M2 TAMs. A CM from MMP28-overexpressing pancreatic cancer cells was treated with an anti-IL-8 neutralizing antibody (5 μg/mL) or an anti-VEGFA neutralizing antibody (1 μg/mL). The migration level of TAMs was determined by the Transwell migration experiment. Scale bar, 100 μm. B qRT-PCR determination of CD86 and CD163 mRNA expression levels in TAMs cocultured with Vector group of pancreatic cancer cells transfected with empty vector virus, TAMs cocultured with OE-MMP28 cell group, TAMs cocultured with the OE-MMP28 + IgG cell group, TAMs cocultured with OE-MMP28 + Anti-IL-8 cell group, and TAMs cocultured with OE-MMP28 + Anti-VEGFA cell group. C The expression levels of the TAM markers CD86 and CD163 in different coculture groups were determined by immunofluorescence staining. Scale bar, 50 μm. D The proportions of CD11b + CD86 + TAMs and CD11b + CD163 + TAMs in different coculture groups were analysed by flow cytometry. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001

Article Snippet: When the tumors reached a volume of at least 80 mm 3 one week post-injection, the treatment groups received the following treatments: PBS, Clodronate Liposomes (200 μL/mouse, YEASEN), the JNK inhibitor SP600125 (15 mg/kg/mouse, MCE), the interleukin-8 (IL-8)-neutralizing antibody MAB208-SP (10 μg/mouse, R&D Systems), or vascular endothelial growth factor A (VEGFA)-neutralizing antibody MAB293-SP (1 μg/mouse, R&D Systems).

Techniques: Migration, Quantitative RT-PCR, Expressing, Plasmid Preparation, Transfection, Virus, Immunofluorescence, Staining, Flow Cytometry

MMP28 enhances M2 TAM infiltration and promotes pancreatic cancer growth in vivo. A Effects of the administration of Clodronate Liposomes, the JNK inhibitor SP600125, the anti-IL-8 neutralizing antibody MAB208-SP, and the anti-VEGFA neutralizing antibody MAB293-SP on subcutaneous tumors in nude mice in the experimental group. B - C Representative images of subcutaneous tumors in nude mice detected with fluorescein potassium salt and the fluorescence quantitative analysis. After the tumor volume reached at least 80mm 3 , PBS, Clodronate Liposomes (200 μg), the JNK inhibitor SP600125 (15 mg/kg), the anti-IL-8 antibody MAB208-SP (10 μg), and the anti-VEGFA antibody MAB293-SP (1 μg) were injected intraperitoneally into the nude mice. D Representative images of subcutaneous xenograft tumors (5 mice per group). E The volume of the tumor was measured every four days. F Tumor weight in both groups. G Representative images of MMP28 expression levels in xenograft tumors from the Vector and OE-MMP28 groups as determined by immunohistochemical staining. Scale bar, 50 μm. H Immunohistochemical staining detection of expression of Ki67 and CD86 + and CD163 + TAM infiltration in xenograft tumors in the Vector group, OE-MMP28 group, OE-MMP28 + SP600125 group, OE-MMP28 + MAB208-SP group, OE-MMP28 + MAB293-SP group, and Vector + Clodronate Liposomes group

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: MMP28 recruits M2-type tumor-associated macrophages through MAPK/JNK signaling pathway-dependent cytokine secretion to promote the malignant progression of pancreatic cancer

doi: 10.1186/s13046-025-03321-x

Figure Lengend Snippet: MMP28 enhances M2 TAM infiltration and promotes pancreatic cancer growth in vivo. A Effects of the administration of Clodronate Liposomes, the JNK inhibitor SP600125, the anti-IL-8 neutralizing antibody MAB208-SP, and the anti-VEGFA neutralizing antibody MAB293-SP on subcutaneous tumors in nude mice in the experimental group. B - C Representative images of subcutaneous tumors in nude mice detected with fluorescein potassium salt and the fluorescence quantitative analysis. After the tumor volume reached at least 80mm 3 , PBS, Clodronate Liposomes (200 μg), the JNK inhibitor SP600125 (15 mg/kg), the anti-IL-8 antibody MAB208-SP (10 μg), and the anti-VEGFA antibody MAB293-SP (1 μg) were injected intraperitoneally into the nude mice. D Representative images of subcutaneous xenograft tumors (5 mice per group). E The volume of the tumor was measured every four days. F Tumor weight in both groups. G Representative images of MMP28 expression levels in xenograft tumors from the Vector and OE-MMP28 groups as determined by immunohistochemical staining. Scale bar, 50 μm. H Immunohistochemical staining detection of expression of Ki67 and CD86 + and CD163 + TAM infiltration in xenograft tumors in the Vector group, OE-MMP28 group, OE-MMP28 + SP600125 group, OE-MMP28 + MAB208-SP group, OE-MMP28 + MAB293-SP group, and Vector + Clodronate Liposomes group

Article Snippet: When the tumors reached a volume of at least 80 mm 3 one week post-injection, the treatment groups received the following treatments: PBS, Clodronate Liposomes (200 μL/mouse, YEASEN), the JNK inhibitor SP600125 (15 mg/kg/mouse, MCE), the interleukin-8 (IL-8)-neutralizing antibody MAB208-SP (10 μg/mouse, R&D Systems), or vascular endothelial growth factor A (VEGFA)-neutralizing antibody MAB293-SP (1 μg/mouse, R&D Systems).

Techniques: In Vivo, Liposomes, Fluorescence, Injection, Expressing, Plasmid Preparation, Immunohistochemical staining, Staining